还想在主楼增加内容？
还想在主楼增加内容？
图片最多上传9张
您的赞赏是对楼主的鼓励！
金额须在1~200元之间
点击图片可以查看大
图呦，亲！求助：麻烦帮忙分析一下 急急急！！_甲状腺吧_百度贴吧
&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&签到排名：今日本吧第个签到，本吧因你更精彩，明天继续来努力！
本吧签到人数：0成为超级会员，使用一键签到本月漏签0次！成为超级会员，赠送8张补签卡连续签到：天&&累计签到：天超级会员单次开通12个月以上，赠送连续签到卡3张
关注：47,434贴子：
求助：麻烦帮忙分析一下 急急急！！收藏
甲状腺左叶宽2.0厘米，厚1.5厘米；甲状腺右叶宽1.6厘米，厚1.5厘米。峡部厚0.2厘米。甲状腺实质回声均匀，可见多个无回声，左侧最大约0.6*0.4厘米，右侧最大约0.7*0.4厘米，边界请，其内可见点状强回声，伴彗星尾征。左叶另可见等回声结节，大笑约2.0*1.4厘米，其内可见多发斑点状及斑块状强回声，周边可见低回声晕，CDI：结节内血流较丰富。CDI：甲状腺内血流分布正常双颈部未探及明显肿大淋巴结请问需要做穿刺什么的吗 基本上是一种什么状况啊
需要做手术吗囊肿 钙化 实质性占位 等是什么意思啊怎样才能确定患病原因啊先谢过了！
做过甲功5项
都在正常范围内
目前也没有什么自觉的不适症状
左边的建议手术。原因有很多，比如不规律的饮食及生活，工作压力大，还有情绪方面。您的报告没有提到囊肿。
能不能通过这个检查结果看出来有没有钙化呀 我做过两次B超 第一次的医院设备有限他们说右侧囊性占位伴钙化 上面的是第二次B超的
现在看这种情况严重不严重呀 需要做穿刺确定是不是良性吗
我有点担心
左边的一定要手术吗
左边那个也有2公分了，从描述看似乎不太理想。如果犹豫不决手术的话，做个穿刺是比较合理的。供参考。
左侧的有钙化，且有手术指征，所以建议手术。
做穿刺是为了确定是良性还是恶性吗？我去的是一家三甲的医院，那个医生当时说基本可以确定是良性，而且不建议手术，说定期检查就行。但是我自己真的不放心。另外，做穿刺对身体其他器官什么的会有伤害吗？
请问钙化具体是什么意思啊？一般出现怎样的情况是指手术指征啊？
钙化的意思就是，由于炎症或其它的原因，造成组织坏死因而形成沉积，这就是钙化。手术指征，一般是超过1CM的结节。但也要视具体情况而定。
那就放心好了，这里的朋友都是远距离查看报告，说白了谁都没有临床经验，而且还有人工检查也很重要，这些都不具备的。那你就定期复查好了，应该没事，放松心情。注意休息。生活规律。
嗯 谢谢您！
我自己再考虑考虑吧 非常感谢！
还想问您一下，如果要做手术是不是手术前要做血常规、尿常规等之类的检查？一般什么情况会不能做手术啊？
术前需要做全血、尿常规、肝功能、甲状腺功能、生化、胸透、心电图。如果有不可控疾病，可能做不了手术。
请问什么是属于不可控疾病呀？像高血压、慢性肝炎、贫血之类的吗？
高血压的波动定期、贫血、肾功能不全、糖尿病的急发期，等。
你好，我想问一下甲状腺里有瘤会引起脖子肿胀吗？这个症状是偏良性还是恶性啊？
是的，结节性甲状腺肿，会有肿胀的情况出现。良恶与否需要手术病理为依据。
登录百度帐号推荐应用
为兴趣而生，贴吧更懂你。或关注今日：10 | 主题：275202
微信扫一扫
扫一扫，下载丁香园 App
即送15丁当
【求助】:大家帮忙分析一下引物！！急急急！！！
页码直达：
这个帖子发布于11年零104天前，其中的信息可能已发生改变或有所发展。
各位老师：
请帮忙看一下我找公司设计的引物，万分感谢。sense:5’CCTGGGTCATAATTGTTGGTG 3'antisense:5’TGGCGGTAATAGGTGTAAATGG3'下面是基因的序列：LOCUS
ROD 23-MAY-2005DEFINITION
Rattus norvegicus intercellular adhesion molecule 1 (Icam1), mRNA.ACCESSION
NM_012967VERSION
GI:6981067KEYWORDS
Rattus norvegicus (Norway rat)
Rattus norvegicus
E M C C V E
S M M Rattus.REFERENCE
(bases 1 to 2602)
Bartsch,M., Weeke-Klimp,A.H., Morselt,H.W., Kimpfler,A.,
Asgeirsdottir,S.A., Schubert,R., Meijer,D.K., Scherphof,G.L. and
Kamps,J.A.
Optimized targeting of polyethylene glycol-stabilized
anti-intercellular adhesion molecule 1 oligonucleotide/lipid
particles to liver sinusoidal endothelial cells
Mol. Pharmacol. 67 (3), 883-890 (2005)
GeneRIF: ICAM-1 antisense oligonuclotides can be targeted to
scavenger receptors on liver endothelial cells by means of
covalently coupled polyanionized albumin.REFERENCE
(bases 1 to 2602)
Hang,C.H., Shi,J.X., Li,J.S., Li,W.Q. and Yin,H.X.
Up-regulation of intestinal nuclear factor kappa B and
intercellular adhesion molecule-1 following traumatic brain injury
World J. Gastroenterol. 11 (8),
GeneRIF: Inflammatory response mediated by increased NF-kappaB
activation and ICAM-1 expression may play an important role in the
pathogenesis of acute gut mucosal injury following traumatic brain
injuryREFERENCE
(bases 1 to 2602)
Zhou,T., Sun,G.Z., Zhang,M.J., Chen,J.L., Zhang,D.Q., Hu,Q.S.,
Chen,Y.Y. and Chen,N.
Role of adhesion molecules and dendritic cells in rat hepatic/renal
ischemia-reperfusion injury and anti-adhesive intervention with
anti-P-selectin lectin-EGF domain monoclonal antibody
World J. Gastroenterol. 11 (7),
GeneRIF: the expression of ICAM-1 was up-regulated in hepatic
sinusoid and renal vessels after 6 hours of ischemia reperfusionREFERENCE
(bases 1 to 2602)
Bonello,N., Jasper,M.J. and Norman,R.J.
Periovulatory expression of intercellular adhesion molecule-1 in
the rat ovary
Biol. Reprod. 71 (4),
GeneRIF: Rat ovarian ICAM-1 may be instrumental in the active
recruitment of leukocytes into the preovulatory ovary and may have
a role in corpus luteum formation.REFERENCE
(bases 1 to 2602)
Davani,E.Y., Dorscheid,D.R., Lee,C.H., van Breemen,C. and
Walley,K.R.
Novel regulatory mechanism of cardiomyocyte contractility involving
ICAM-1 and the cytoskeleton
Am. J. Physiol. Heart Circ. Physiol. 287 (3), H (2004)
GeneRIF: Cardiomyocyte ICAM-1 binding, by polymorphonuclear
leukocytes or other ligands, induces decreased cardiomyocyte
contractility via this pathway.REFERENCE
(bases 1 to 2602)
Lu,L., Zhang,J.Q., Ramires,F.J. and Sun,Y.
Molecular and cellular events at the site of myocardial infarction:
from the perspective of rebuilding myocardial tissue
Biochem. Biophys. Res. Commun. 320 (3), 907-913 (2004)
GeneRIF: We found that enhanced ICAM-l and MCP-1, as well as MMP
activity on day 3 and 7 postMI, are present to facilitate the
homing, chemotaxis, and migration of circulating cells into the
infarct site.REFERENCE
(bases 1 to 2602)
Wang,H., Nawata,J., Kakudo,N., Sugimura,K., Suzuki,J., Sakuma,M.,
Ikeda,J. and Shirato,K.
The upregulation of ICAM-1 and P-selectin requires high blood
pressure but not circulating renin-angiotensin system in vivo
J. Hypertens. 22 (7),
GeneRIF: high blood pressure, but not the circulating
renin-angiotensin system, upregulates the expression of ICAM-1 and
P-selectinREFERENCE
(bases 1 to 2602)
Nordal,R.A. and Wong,C.S.
Intercellular adhesion molecule-1 and blood-spinal cord barrier
disruption in central nervous system radiation injury
J. Neuropathol. Exp. Neurol. 63 (5), 474-483 (2004)
GeneRIF: The parallel dose response, time course, and spatial
distribution of ICAM-1 expression and albumin leakage suggest a
role for ICAM-1 in late BSCB disruption after radiation.REFERENCE
(bases 1 to 2602)
Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
Tonellato,P.J. and Jacob,H.J.
High-density rat radiation hybrid maps containing over 24,000
SSLPs, genes, and ESTs provide a direct link to the rat genome
Genome Res. 14 (4), 750-757 (2004)
10 (bases 1 to 2602)
El-Wahsh,M., Fuller,B., Davidson,B. and Rolles,K.
Hepatic cold hypoxia and oxidative stress: implications for ICAM-1
expression and modulation by glutathione during experimental
isolated liver preservation
Cryobiology 47 (2), 165-173 (2003)
GeneRIF: liver ICAM-1 expression is demonstrably increased by
progressive cold preservation and reperfusionREFERENCE
11 (bases 1 to 2602)
De Greef,K.E., Ysebaert,D.K., Persy,V., Vercauteren,S.R. and De
ICAM-1 expression and leukocyte accumulation in inner stripe of
outer medulla in early phase of ischemic compared to HgCl2-induced
Kidney Int. 63 (5),
GeneRIF: very early after ischemia reperfusion injury, increased
expression of ICAM-1 goes along with monocyte and T-cell
accumulation in the inner stripe of outer kidney medullaREFERENCE
12 (bases 1 to 2602)
Romero,I.A., Amos,C.L., Greenwood,J. and Adamson,P.
Ezrin and moesin co-localise with ICAM-1 in brain endothelial cells
but are not directly associated
Brain Res. Mol. Brain Res. 105 (1-2), 47-59 (2002)
GeneRIF: The ERM proteins ezrin and moesin were found to strongly
co-localise with both ICAM-1 and F-actin in brain microvascular
endothelial cells suggesting a potential role in mediating ICAM-1
signalling.REFERENCE
13 (bases 1 to 2602)
Bowen-Yacyshyn,M.B., Bennett,C.F., Nation,N., Rayner,D. and
Yacyshyn,B.R.
Amelioration of chronic and spontaneous intestinal inflammation
with an antisense oligonucleotide (ISIS 9125) to intracellular
adhesion molecule-1 in the HLA-B27/beta2 microglobulin transgenic
J. Pharmacol. Exp. Ther. 302 (3), 908-917 (2002)
GeneRIF: regulatory role of ICAM-1 in chronic intestinal
inflammationREFERENCE
14 (bases 1 to 2602)
Hofmann,N., Lachnit,N., Streppel,M., Witter,B., Neiss,W.F.,
Guntinas-Lichius,O. and Angelov,D.N.
Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by
spinal perivascular macrophages during experimental allergic
encephalomyelitis in rats
(er) BMC Immunol. 3 (1), 11 (2002)
GeneRIF: During experimental allergic encephalomyelitis, a strong
activation of spinal perivascular macrophages is observed
accompanied by a consistently increased expression of ICAM-1.REFERENCE
15 (bases 1 to 2602)
Lu,Y.T., Chen,P.G. and Liu,S.F.
Time course of lung ischemia-reperfusion-induced ICAM-1 expression
and its role in ischemia-reperfusion lung injury
J. Appl. Physiol. 93 (2), 620-628 (2002)
GeneRIF: ICAM-1 upregulation is an important mechanism of
I/R-induced pulmonary endothelial injury.REFERENCE
16 (bases 1 to 2602)
Haddad,el.-B., McCluskie,K., Birrell,M.A., Dabrowski,D.,
Pecoraro,M., Underwood,S., Chen,B., De Sanctis,G.T., Webber,S.E.,
Foster,M.L. and Belvisi,M.G.
Differential effects of ebselen on neutrophil recruitment,
chemokine, and inflammatory mediator expression in a rat model of
lipopolysaccharide-induced pulmonary inflammation
J. Immunol. 169 (2), 974-982 (2002)
17 (bases 1 to 2602)
Machelska,H., Mousa,S.A., Brack,A., Schopohl,J.K., Rittner,H.L.,
Schafer,M. and Stein,C.
Opioid control of inflammatory pain regulated by intercellular
adhesion molecule-1
J. Neurosci. 22 (13),
18 (bases 1 to 2602)
Masamune,A., Sakai,Y., Kikuta,K., Satoh,M., Satoh,A. and
Shimosegawa,T.
Activated rat pancreatic stellate cells express intercellular
adhesion molecule-1 (ICAM-1) in vitro
Pancreas 25 (1), 78-85 (2002)
GeneRIF: activated pancreatic stellate cells express ICAM-1 mainly
through the activation of NF-kappa B, thus playing a role in the
pathogenesis of pancreatic inflammation.REFERENCE
19 (bases 1 to 2602)
Olanders,K., Sun,Z., Borjesson,A., Dib,M., Andersson,E., Lasson,A.,
Ohlsson,T. and Andersson,R.
The effect of intestinal ischemia and reperfusion injury on ICAM-1
expression, endothelial barrier function, neutrophil tissue influx,
and protease inhibitor levels in rats
Shock 18 (1), 86-92 (2002)
GeneRIF: intestinal ischemia and reperfusion injury results in an
increase of systemic ICAM-1 expression with marked organ
variabilityREFERENCE
20 (bases 1 to 2602)
Lu,S., Wang,X., Wen,L., Han,Z., Tao,T. and Guan,D.
[Effect of superoxide dismutase on adhesion molecules expression in
skeletal muscle ischemia/reperfusion injury in rats]
Zhonghua Yi Xue Za Zhi 82 (12), 840-843 (2002)
GeneRIF: study the effect of SOD on the expression of CD11b/CD18
and ICAM-1 after skeletal muscle ischemia / reperfusion injury in
ratsREFERENCE
21 (bases 1 to 2602)
Weigel,J.A., Raymond,R.C. and Weigel,P.H.
The hyaluronan receptor for endocytosis (HARE) is not CD44 or CD54
Biochem. Biophys. Res. Commun. 294 (4), 918-922 (2002)
22 (bases 1 to 2602)
Yasuda,M., Shimizu,S., Ohhinata,K., Naito,S., Tokuyama,S., Mori,Y.,
Kiuchi,Y. and Yamamoto,T.
Differential roles of ICAM-1 and E-selectin in polymorphonuclear
leukocyte-induced angiogenesis
Am. J. Physiol., Cell Physiol. 282 (4), C917-C925 (2002)
GeneRIF: These findings suggested that ICAM-1 in endothelial cells
may act as a signaling receptor to induce Ets-1 expression in
angiogenesisREFERENCE
23 (bases 1 to 2602)
Joussen,A.M., Poulaki,V., Qin,W., Kirchhof,B., Mitsiades,N.,
Wiegand,S.J., Rudge,J., Yancopoulos,G.D. and Adamis,A.P.
Retinal vascular endothelial growth factor induces intercellular
adhesion molecule-1 and endothelial nitric oxide synthase
expression and initiates early diabetic retinal leukocyte adhesion
Am. J. Pathol. 160 (2), 501-509 (2002)
GeneRIF: retinal expression induced by VEGFREFERENCE
24 (bases 1 to 2602)
Donadio,A.C., Remedi,M.M., Frede,S., Bonacci,G.R., Chiabrando,G.A.
and Pistoresi-Palencia,M.C.
Decreased expression of intercellular adhesion molecule-1 (ICAM-1)
and urokinase-type plasminogen activator receptor (uPAR) is
associated with tumor cell spreading in vivo
Clin. Exp. Metastasis 19 (5), 437-444 (2002)
GeneRIF: Decreased expression of urokinase-type plasminogen
activator receptor (uPAR) is associated with tumor cell spreading
in vivo.REFERENCE
25 (bases 1 to 2602)
Kiarash,A., Pagano,P.J., Tayeh,M., Rhaleb,N.E. and Carretero,O.A.
Upregulated Expression of Rat Heart Intercellular Adhesion
Molecule-1 in Angiotensin II- but Not Phenylephrine- Induced
Hypertension
Hypertension 37 (1), 58-65 (2001)
26 (bases 1 to 2602)
Kita,Y., Takashi,T., Iigo,Y., Tamatani,T., Miyasaka,M. and
Horiuchi,T.
Sequence and expression of rat ICAM-1
Biochim. Biophys. Acta 1131 (1), 108-110 (1992)
1349828COMMENT
PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from D00913.1.
SUMMARY: ce ligand for leukocyte adhesion
molecule LFA-1 [RGD].FEATURES
Location/Qualifiers
/organism=&Rattus norvegicus&
/mol_type=&mRNA&
/db_xref=&taxon:10116&
/chromosome=&8&
/map=&8q13&
/gene=&Icam1&
/note=&synonyms: CD54, ICAM&
/db_xref=&GeneID:25464&
/db_xref=&RATMAP:41349&
/db_xref=&RGD:2857&
/gene=&Icam1&
/note=&go_component: membrane [goid 0016020] [evidence
go_component: plasma membrane [goid 0005886] [evidence
go_component: integral to membrane [goid 0016021]
[evidence IEA];
go_function: protein binding [goid 0005515] [evidence
go_process: cell adhesion [goid 0007155] [evidence IEA];
go_process: defense response [goid 0006952] [evidence
go_process: cell-cell adhesion [goid 0016337] [evidence
/codon_start=1
/product=&intercellular adhesion molecule 1&
/protein_id=&NP_&
/db_xref=&GI:6981068&
/db_xref=&GeneID:25464&
/db_xref=&RATMAP:41349&
/db_xref=&RGD:2857&
/translation=&MASTRARPMLPLLLVLVAVVIPGPVGAQVSIHPTEAFLPRGGSV
QVNCSSSCEDENLGLGLETNWMKDELSSGHNWKLFKLSDIGEDSRPLCFENCGTTQSS
ASATITVYSFPERVELDPLPAWQQVGKNLILRCLVEGGAPRTQLSVVLLRGNETLSRQ
AVDGDPKEITFTVLASRGDHGANFSCFTELDLRPQGLSLFKNVSEVRQLRTFDLPTRV
LKLDTPDLLEVGTQQKFLCSLEGLFPASEAQIYLEMGGQMLTLESTNSRDFVSATASV
EVTEKLDRTLQLRCVLELADQTLEMEKTLRIYNFSAPILTLSQPEVSEGDQVTVKCEA
HGGAQVVLLNSTSPRPPTSQGTSPRPPTSQIQFTLNASPEDHKRRFFCSAALEVDGKS
LFKNQTLELHVLYGPHLDKKDCLGNWTWQEGSQQTLTCQPQGNPAPNLTCSRKADGVP
LPIGMVKSVKREMNGTYKCRAFSSRGSITRDVHLTVLYHDQNTWVIIVGVLVLIIAGF
VIVASIYTYYRQRKIRIYKLQKAQEEALKLKVQAPPP&
polyA_signal
/gene=&Icam1&ORIGIN
1 ctgctgcctg cactttgccc tggtcctcca atggcttcaa cccgtgccag gcccatgctg
61 cctctgctcc tggtcctggt cgccgttgtg atccccgggc ctgtcggtgc tcaggtatcc
121 atccatccca cagaagcctt cctgcctcgg ggtggatccg tgcaggtgaa ctgctcttcc
181 tcttgcgaag acgagaacct cggcctgggg ttggagacta actggatgaa agacgaacta
241 tcgagtggac acaactggaa gctcttcaag ctgagcgaca ttggggaaga cagcagacca
301 ctgtgctttg agaactgtgg caccacgcag tcctcggctt ctgccaccat cactgtgtat
361 tcgttcccag agcgagtgga gctggatcct ctgcccgcct ggcagcaggt gggcaagaac
421 ctcatcctgc gctgcctggt ggaaggcgga gcaccgcgga cacagctctc agtagtgctg
481 ctccgtggga atgagacact gagccgccag gcagtggatg gggaccccaa ggagatcaca
541 ttcacggtgc tggccagcag aggcgaccac ggagccaatt tctcatgctt cacagaactg
601 gacctcaggc cacaagggct gtcactgttc aagaatgtct ccgaggtcag gcagctccgg
661 actttcgatc ttccgactag ggtcctgaag ctcgacaccc ctgacctcct ggaggtgggc
721 acccagcaga agttcttgtg ttccctggaa ggcctgtttc ctgcctctga agctcagata
781 tacctggaga tgggaggcca gatgctgacc ctggagagca caaacagcag agattttgtg
841 tcagccactg cctcagtgga ggtgactgag aagttggaca gaaccctgca gctgcgctgt
901 gttttggagc tggcggacca gaccctggag atggagaaga ccttgagaat ctacaacttt
961 tcagctccca tcctgaccct gagccagccg gaggtctcag aaggggacca agtaactgtg
1021 aagtgtgaag cccacggtgg ggcacaggtg gtgcttctga acagtacttc ccccaggcca
1081 cccacctcac agggtacttc ccccaggcca cccacctcac agatccaatt cacactgaat
1141 gccagcccgg aggatcacaa acgacgcttc ttttgctctg cggccttgga ggtggatggg
1201 aagtccctgt ttaaaaacca gaccttggaa ctccatgtgc tatatggtcc tcacctggac
1261 aagaaggact gcttggggaa ctggacctgg caagaggggt ctcagcagac tcttacatgc
1321 cagccccagg ggaatccagc ccctaatctg acctgcagcc ggaaagcaga tggtgtcccg
1381 ctgcctatcg ggatggtgaa gtctgtcaaa cgggagatga atggtaccta caagtgccgt
1441 gcctttagct cccgtgggag tatcaccagg gacgtgcacc tgacagtgct gtaccatgat
1501 cagaatacct gggtcataat tgttggtgtg ttggtactga tcattgcggg cttcgtgatc
1561 gtggcgtcca tttacaccta ttaccgccag aggaagatca ggatatacaa gttacagaag
1621 gctcaggagg aggccctaaa actcaaggta caagccccgc ctccctgagc ccactggaca
1681 ggacacctgc ctgggccccg ctgctcttga acagatcaat ggacagcatt tacccctcac
1741 ccacctcctc tggctgtcac aggacaggac agtggcctgg ggatgcatac ttgtagcctc
1801 aggcctaaga ggactcggag gggcaagact gtgaactcgt gacctggaca cacctacagc
1861 ctggtgggcc tgcagccaag aaaggctgac ttccttctct attacccctg ctgaggggcc
1921 ccctacctta ggaaggtgtg atatccggta gacacaagca agagaagaaa aggaacacca
1981 tgcttcctct gacatgggaa agctgggaca ctgtccccaa ctcttgttga tgtatttatt
2041 aattcagagt tctgacagtt atttattgag taccctgtac agacactaga ggagtgagca
2101 ggttaacatg taagttattg cctagaccct ggtgaagggg cacaacagag tctggggaaa
2161 gatcatacgg gtttgggctt ctccacaggt cagggtgctt tcctcaaaag agctgatttc
2221 tttcacgagt catataaata ctatgtggac gagcagtggc cctctgctcg tagacctctc
2281 tgggacccct gcctcctccc acagcctgga gtctcccagc accagcatgg gtgaccacct
2341 ccccacctac atacattcct acctttgttc ccaatgtcaa ccaccatgcc taaatatgga
2401 cgctcacctt tagcagctca acaatggagt ctcatgcccg tgaaattatg gtcaatccct
2461 gcatgcctcc acccggctcc acctcaaaga gaatgcctgg gagaaaatgt tccaaccact
2521 tagaagggtc ctgcaagctg ttgtgggagg gtaggcaccc ctcccagcgc agaagccttt
2581 cctttgaatc aataaagttt ta//
不知道邀请谁？试试他们
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
没有人回吗，自己顶
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
真的没有人回吗
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
理论上看引物设计没有什么问题，不过只扩增80bp左右的片断，检测上是不是会有一些问题
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
可以帮忙用软件分析一下吗？多谢了
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
真的没有愿意帮忙的吗
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
可以帮忙用软件分析一下吗
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
序列已纪分析，1. 如zjwang77 所说，你的序列太短了只有81个bp，不是太好。如图所示，你引物间可能形成引物二聚体，这样和你的目的片断恐怕很难分开。2. 下游引物可能形成错配，且该错配可能会形成产物，干扰你的正常目的条带。3. 如你所示，你做的是RT，最好是跨外显子设计引物，你的片断如此之短，恐怕也很难满足以上要求。
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
看看这对引物：Sense： CAAACGGGAGATGAATGGAntisense： ACGAAGCCCGCAATG退火温度、扩增片断长度等见下图：只有一处cross dimer，问题并不严重。唯一的缺点是退火温度稍低了些，不过做RT-PCR应该可以用。
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
笑佛 edited on
关于丁香园　　本人男，上海打工的。目前有两个工作需要做出选择。一个是第三方理财机构，本人积累有客户资源。朋友介绍。第二个工作是银行个人无抵押贷款。也是朋友介绍的。本人倾向做一个有前途的职业，希望懂这块的人帮忙指点一下那个工作更有前途。目前两个工作待遇差不多。
楼主发言：1次 发图：0张
　　貌似都是骗子公司嘛
　　@Nazi卩灬鹰
21:07:00　　貌似都是骗子公　　不是的，银行是百年老银行，理财机构在国内也很知名。
　　如果是中资银行建议去银行，至少是国企，有保障。
21:14:00　　如果是中资银行建议去银行，至少是国企，有保障。 　　-----------------------------　　额，是外资银行。。。
　　。。。
　　在线等回复
请遵守言论规则，不得违反国家法律法规