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African Journal of Neurological Sciences
Les techniques actuelles d’etude de l’anatomie de la substance blanche du systeme nerveux central
Dominique Ndri Oka, Nicole Adou, Koffi Fulbert Kouakou, Stéphane Velut
L’étude des fibres de la substance blanche de l’encéphale demeure complexe. Pour ce faire plusieurs méthodes ont été utilisées pour mieux comprendre la disposition de la micro anatomie de ces fibres. La technique de dissection des fibres ex vivo f?t la première technique .Elle a ensuite été abandonnée au profit du microtome et de l’histologie du fait des difficultés pratiques engendrées. C’est à partir de 1956 qu’elle a connu un regain d’intérêt avec son amélioration par Josef Klingler. A partir des années 2000 alors que la technique continue d’être améliorée, une autre technique de dissection in vivo est mise au point. Les méthodes de d’étude de la substance blanche de l’encéphale se résument à la dissection selon la technique de Klingler et la tractographie réalisée par l’IRM en tenseur de diffusion.
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, 15 July 2014, Pages 522–526
Variations in fluorescence quantum yield of basic fuchsin with silver nanoparticles prepared by femtosecond laser ablation, , , , , , , , , , , a International School of Photonics, Cochin University of Science and Technology, Cochin, Kerala, Indiab Department of Microbiology, Medical College, Thiruvananthapuram, Kerala, Indiac LBS Centre for Science and Technology, Thiruvananthapuram, Kerala, India&Silver sol is prepared by femtosecond laser.&Absolute fluorescence quantum yield is calculated using thermal lens technique.&Quantum yield variations of dye with and without silver sol are plotted.&The presence of silver sol decreases the fluorescence quantum efficiency.&The presence of silver sol enhances the thermal lens signal.Nano structured noble metals have very important applications in diverse fields such as photovoltaics, catalysis, electronic and magnetic devices, etc. In the present work, the application of dual beam thermal lens technique is employed for the determination of the absolute fluorescence quantum yield of the triaminotriphenylmethane dye, basic fuchsin in the presence of silver sol is studied. Silver sol is prepared by femtosecond laser ablation. It is observed that the presence of silver sol decreases the fluorescence quantum efficiency. The observed results are in line with the conclusion that the reduction in quantum yield in the quenching region is essentially due to the non-radiative relaxation of the absorbed energy. It is also observed that the presence of silver sol enhances the thermal lens signal which makes its detection easier at any concentration.Graphical abstractKeywordsBasic fuchsin; Thermal lens spectroscopy; Fluorescence quantum yield; Silver nanoparticles; Femtosecond laser; Laser ablation
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